Figure 2.

Plasma lipoprotein analysis of LDLRKO mice that received various diets. Pooled plasma samples from 5 mice/diet group that received chow or chow + HDCA diets for 3 wk were used for FPLC analysis. A–C) FPLC fractions were used to quantify total cholesterol (A), phospholipid (B), and triglyceride (C) concentrations. See Table 6 for lipid composition of VLDL and IDL/LDL from diet groups. D) The apoB-48, apoB-100, and apoE contents of the VLDL fractions (lanes 12–15) and LDL fractions (lanes 24–27) collected from FPLC were examined by immunoblotting as described in Materials and Methods.