Figure 5.

Examination of HDL properties and cholesterol efflux ability of HDL isolated from LDLRKO mice that received various diets, and the effect of HDCA treatment on expression of genes involved in cholesterol efflux in RAW 264.7 cells. A–D) HDL samples were isolated from pooled plasma of mice fed the chow or chow + HDCA diets by FPLC and used in immunoblotting using an antibody against mouse apoA1 (A), determination of total bile acid levels in HDL and plasma (B), examination of extent of lipid oxidation by the DCF assay (C), and cholesterol efflux assay as described in Materials and Methods (D). *P < 0.05, **P < 0.01, ***P < 0.0001 vs. chow diet group. E) Gene expression analysis of RAW 264.7 cells treated with DMSO (vehicle) or 50 or 100 μM of HDCA for 24 h. *P < 0.05, **P < 0.01, ***P < 0.0001 vs. DMSO group.