Figure 1.
Generation of mKO mice. A) Schematic diagram of the Sod1 genomic construct and the recombination events in the generation of Sod1flox/flox mice. E1–E5, Sod1 exon 1 to 5; probe 1 and probe 2, DNA probes for Southern blot analysis to confirm the recombination between genomic DNA and the Cre-Lox/Sod1 construct; TK, thymidine kinase; Neo, neomycin resistance; Frt, flipase recognition target; LoxP, recombination site recognized by Cre recombinase. B) Southern blot analysis of the integration of Cre-Lox/Sod1 constructs into mouse genome. C) Western blot analysis of Sod1 expression in various tissues of mKO mice. D) Western blot analysis of Sod1 expression in multiple skeletal muscles in mKO mice. E) Enzymatic activity analysis of CuZnSOD.