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Western blot analysis of the reconstituted fibers to estimate the level of incorporation of McTnT proteins. Reconstituted fibers containing TmWT background were solubilized using 2% SDS (26). Solubilized fiber samples were run on a 10% SDS gel and were then transferred onto a PVDF membrane for Western blot analysis. Lane 1 shows purified recombinant McTnT protein. Lanes 2–4 show samples from McTnTWT, McTnT1–44Δ, and McTnT45–74Δ reconstituted fiber samples, respectively.
