Figure 2.
Effect of McTnT deletion mutants on Ca2+-activated maximal tension in TmWT and TmH276N fibers. Ca2+-activated maximal tension was measured at pCa 4.3. A) Two-way ANOVA revealed a significant Tm-cTnT interaction effect (P<0.05), indicating that the differences between the effects of McTnT1–44Δ and McTnT45–74Δ were dissimilar against TmWT and TmH276N backgrounds. B) Subsequent post hoc multiple pairwise comparisons (Fischer's LSD) revealed that differential effect of McTnT45–74Δ on tension against TmWT and TmH276N was responsible for the interaction effect. Specifically, McTnT45–74Δ decreased maximal tension in TmH276N fibers by ∼19% (P<0.01), but not in TmWT fibers. On the other hand, McTnT1–44Δ decreased tension against TmWT (∼36%) and TmH276N (∼38%) uniformly. At least 7 determinations were done for each group. Values are reported as means ± sem. **P < 0.01; ***P < 0.0001.