Figure 6.
Effect of McTnT deletion mutants on the pCa-tension relationship in TmWT and TmH276N fibers. Ca2+-activated tension was measured at the SL of 2.3 μm. Normalized tension values were plotted against a range of pCa to derive the pCa-tension relationships. Normalized pCa-tension relationships were fitted using the Hill equation; the fitted curves are shown as connecting lines. A) Effect of McTnT deletion mutants on the pCa-tension relationships in TmWT fibers. B) Effect of McTnT deletion mutants on the pCa-tension relationships in TmH276N fibers. At least 7 determinations were done for each group. Values are reported as means ± sem.