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. 2013 Sep;27(9):3848–3859. doi: 10.1096/fj.13-232363

Figure 7.

Figure 7.

Effect of McTnT deletion mutants on myofilament cooperativity, nH, and myofilament Ca2+ sensitivity, pCa50, in TmWT and TmH276N fibers. The Hill equation was fitted to the pCa-tension relationships to estimate nH and pCa50 values. A) Two-way ANOVA revealed no significant Tm-cTnT interaction effect (P=0.98) on nH. B) Subsequent post hoc multiple pairwise comparisons revealed a significant main effect (P<0.05) of McTnT deletion mutants on nH. Post hoc tests revealed that McTnT45–74Δ decreased nH significantly (P<0.001) in TmWT and TmH276N fibers, respectively. C) Two-way ANOVA revealed no significant Tm-cTnT interaction effect (P=0.63) on pCa50. D) Subsequent post hoc multiple pairwise comparisons revealed significant main effects of McTnT deletion mutants (P<0.0001) and Tm variants (P<0.05) on pCa50. Post hoc tests revealed that McTnT1–44Δ significantly decreased pCa50, while McTnT45–74Δ significantly increased pCa50 in TmWT and TmH276N fibers. At least 7 determinations were done for each group. Values are reported as means ± sem. **P < 0.01; ***P < 0.001.