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. 2013 Sep;27(9):3848–3859. doi: 10.1096/fj.13-232363

Table 1.

Steady-state contractile parameters of NTG (TmWT) and TG (TmH276N) cardiac muscle fibers reconstituted with the WT Tn complex

Parameter McTnTWT + McTnI + McTnC reconstituted into NTG TmWT cardiac muscle fibers McTnTWT + McTnI + McTnC reconstituted into TG TmH276N cardiac muscle fibers
Tension (mN·mm−2) 49.75 ± 0.94 53.19 ± 1.82
ATPase (pmol·mm−3·s−1) 304.38 ± 9.36 274.53 ± 7.42
nH tension 2.62 ± 0.13 2.94 ± 0.26
pCa50 tension 5.65 ± 0.01 5.63 ± 0.01
Tension cost (pmol·mN−1·mm−1·s−1) 6.39 ± 0.18 5.17 ± 0.15**
ktr (s−1) 12.87 ± 0.46 13.38 ± 0.57

Isometric steady-state tension and ATPase activity were measured simultaneously, as described previously (22, 27). The Hill equation was fitted to the pCa-tension relationships to derive nH- and pCa50-tension values. Tension cost was estimated as described previously (23). ktr was measured according to a protocol described previously (31). At least 7 determinations were done for each group. Values are reported as means ± sem

**

P < 0.01 vs. TmWT.