Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of incubation time on S-nitrosothiol reduction in whole fixed cells. Cellular S-nitrosylation was induced by incubation of COS 7 cells with 200 μM CSNO for 15 minutes. Cells were fixed with methanol and blocked with NEM. 2 mM PE SO3 was added for the time indicated to reduce S-nitrosothiols and reduced thiols labeled with Alexa Fluor 488 C5 maleimide.
