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UV photolysis of protein nitrosothiols in whole fixed cells. Cellular protein S-nitrosylation was induced by incubation of COS 7 cells with CSNO as indicated. After treatment, cells were rinsed and fixed and then either kept in the dark or exposed to UV light to photolyze SNO bonds. After 5 minutes, cells were blocked with NEM, reduced by 2 mM PE SO3 for 30 minutes and then labeled with Alexa Fluor 488 C5 maleimide.
