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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Microbiol. 2013 Jun 24;89(2):372–387. doi: 10.1111/mmi.12282

Figure 5.

Figure 5

Gel shift assay was performed by incubating wild type and three DNA variants of the 398 bp PfasMT promoter region (Mut1, Mut2 and Mut3) with His6-FasRMT in the presence of poly-dIdC. In Mut1, both IRs present in the protected region 1 were replaced by the random sequence 5′-CGAATTATGAGCTCGTAACATGAGC-3′; in Mut2, the IR present in the protected region 2 was replaced by the random sequence 5′-CGAATTATGAGC-3′ and in Mut3, the three IRs identified were simultaneously replaced by the same random sequences used in Mut1 and Mut2.