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. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: Pediatr Res. 2010 Feb;67(2):144–149. doi: 10.1203/PDR.0b013e3181c2df4f

Figure 4.

Figure 4

Nitrotyrosine levels in lung (A) and liver (B) tissues from newborn mouse pups exposed to RA (white hatched bars) or >95% O2 (gray bars). Snap-frozen tissues were homogenized and treated with dinitrophenyl hydrazine. Samples were hybridized to nitrocellulose membranes using a slot blot followed by incubation with anti-dinitrophenyl hydrazine antibodies as described in Methods. Immunoreactive bands were quantitated by densitometry. Results are reported as mean ± SEM, and data were assessed by two-way ANOVA with modified t test posthoc for individual differences, n = 5, p < 0.05. An effect of exposure and an interaction were detected on the lung tissues. An effect of day was detected on liver tissues. *Indicates different from same day RA.