Lipid levels in liver tissues from newborn mouse pups exposed to RA (white hatched bars) or >95% O2 (gray bars). Snap-frozen tissues were extracted and lipids were analyzed by LC-MS/MS as described in Methods. Total HETE levels were the sum of 5-, 8-, 9-, 11-, and 12-HETE measurements (A). Total prostaglandin levels represent the sum of prostaglandin D2, E2, F2α, and thromboxane B2 measurements (B). Results are reported as mean ± SEM, and data were assessed by two-way ANOVA with modified t test post hoc for individual differences, n = 5 to 7, p < 0.05. An effect of exposure was observed on total hepatic HETE levels, and an effect of day and an effect of exposure were observed on total prostaglandin levels. *Indicates different from same day RA.