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Chromatograms from negative control vs. siRNA treated cell populations. Representative chromatograms from sequencing of cDNA samples derived from RNA isolated 72h post-transfection with: (I) 0.6µg negative control siRNA and (II) 0.4µg si_A1_i from the time-course study. Arrows mark the position for the COL1A1 rs3840780 insertion and the asterisks mark the peak-pairs used to determine allele ratios using PeakPicker software.
