Table 1.

PCR primer sequences and reaction conditions were used for the amplification of sequences encoding P. falciparum pfcrt and pfmdr-I gene

Primer name	Primer sequence	PCR product size (bp)	PCR cycling conditions
pfcrt
K76T F (sense)	5′-TGTGCTCATGTGTTTAAACTT-3′	134	95°C for 5 minutes; 30 cycles of 95°C for 30 seconds, 50°C for 30 seconds, and 72°C for 1 minute; 72°C for 4 minutes
K76T R (antisense)	5′-CAAAACTATAGTTACCAATTTTG-3′	134
pfmdr-I
N86Y F (sense)	5′-TTTACCGTTTAAATGTTTACCTGC-3′	310	95°C for 5 minutes; 40 cycles of 95°C for 30 seconds, 56°C for 35 seconds, and 72°C for 1 minute; 72°C for 5 minutes
N86Y R (antisense)	5′-CCATCTTGATAAAAAACACTTCTT-3′	310
N1042D F (sense)	5′-TATGTCAAGCGGAGTTTTTGC-3′	337	95°C for 5 minutes; 42 cycles of 95°C for 30 seconds, 56°C for 40 seconds, and 72°C for 1 minute; 72°C for 5 minutes
N1042D R (antisense)	5′-TCTGAATCTCCTTTTAAGGAC-3′	337
D1246Y F (sense)	5′-GTGGAAAATCAACTTTTATGA-3′	499	95°C for 5 minutes; 44 cycles of 95°C for 30 seconds, 58°C for 40 seconds, and 72°C for 1 minute; 72°C for 8 minutes
D1246Y R (antisense)	5′-TTAGGTTCTCTTAATAATGCT-3′	499