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Ethanol precipitation of exopolysaccharides from a P. aeruginosa biofilm exposed to imipenem (1.0 μg/ml) (left) compared to that for a nonexposed biofilm (right). The biofilm was exposed to imipenem for 24 h. Uronic acid assay measurements verified the increased levels of production of uronic acids from the imipenem-exposed P. aeruginosa biofilm, showing an approximately 20-fold higher level of uronic acids.
