Table 3.
Prevalence of Trypanosoma cruzi infection determined by xenodiagnosis and kDNA-PCR in sylvatic mammals from Pampa del Indio, Chaco, Argentina, 2008–2011*
| Species | % Infection prevalence (no. examined) | Positive by both methods | Positive only by xenodiagnosis | Positive only by kDNA-PCR | Negative by both methods | ||
|---|---|---|---|---|---|---|---|
| Xenodiagnosis | kDNA-PCR | Composite | |||||
| Dasypus novemcinctus | 48.0 (25) | 56.0 (25) | 57.7 (26)† | 11 | 0 | 3‡ | 11 |
| Tolypeutes matacus | 12.5 (16) | 0.0 (16) | 12.5 (16) | 0 | 2 | 0 | 14 |
| Chaetophractus vellerosus | 6.3 (16) | 6.3 (16) | 6.2 (16) | 1 | 0 | 0 | 15 |
| Euphractus sexcinctus | 0.0 (5) | 20.0 (5) | 20.0 (5) | 0 | 0 | 1§ | 4 |
| Chaetophractus villosus | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Didelphis albiventris | 29.3 (41) | 35.7 (42) | 38.1 (42)† | 11 | 1 | 4¶ | 25 |
| Thylamys pusilla | 0.0 (20) | 25.0 (20) | 25.0 (20) | 0 | 0 | 5# | 15 |
| Monodelphis sp. | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Unidentified small rodents | 0.0 (37) | 5.7 (35) | 5.4 (37)** | 0 | 0 | 2†† | 33 |
| Galea musteloides | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Ctenomys sp. | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Nasua nasua | 0.0 (1) | 0.0 (4) | 0.0 (4)§§ | 0 | 0 | 0 | 1 |
| Procyon cancrivorus | 0.0 (4) | 0.0 (4) | 0.0 (4) | 0 | 0 | 0 | 4 |
| Cerdocyon thous | 0.0 (4) | 0.0 (4) | 0.0 (4) | 0 | 0 | 0 | 4 |
| Leopardus geoffroyi | 0.0 (3) | 0.0 (3) | 0.0 (3) | 0 | 0 | 0 | 3 |
| Myotis sp. | 0.0 (2) | 0.0 (2) | 0.0 (2) | 0 | 0 | 0 | 2 |
| Eumops perotis | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Dasypterus ega | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Molossops temminckii | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Sylvilagus brasiliensis | 0.0 (3) | 0.0 (3) | 0.0 (3) | 0 | 0 | 0 | 3 |
| Myrmecophaga tridactyla | 0.0 (1) | 0.0 (1) | 0.0 (1) | 0 | 0 | 0 | 1 |
| Total | 14.6 (185) | 20.3 (187) | 22.1 (190) | 23 | 3 | 15 | 142 |
kDNA = kinetoplast DNA; PCR = polymerase chain reaction.
Includes one negative sample evaluated only by kDNA-PCR.
Two negative samples (1 positive by kDNA-PCR from bugs tested by xenodiagnosis) and 1 positive sample by nuclear satellite DNA-PCR.
One negative sample by nuclear satellite DNA-PCR and positive by kDNA-PCR from bugs tested by xenodiagnosis.
Three negative samples (2 positive by kDNA-PCR from bugs tested by xenodiagnosis) and 1 positive by nuclear satellite DNA-PCR.
Three negative samples (1 positive by kDNA-PCR from bugs tested by xenodiagnosis and 1 with two positive results by kDNA from a blood-guanidine mixture in different DNA extractions) and two positive by nuclear satellite DNA-PCR.
Includes two negative samples evaluated only by xenodiagnosis.
Two negative samples by nuclear satellite DNA-PCR (both positive by kDNA-PCR from bugs tested by xenodiagnosis).
Includes three negatives samples evaluated only by kDNA-PCR.