Figure 4.

Detection of specific anti-ROP8 antibodies in sera of mice immunized with ROP8-pVAX-1, by western blotting using Toxoplasma gondii antigen. Reactivity of sera from mice immunized with, ROP8-pVAX-1 (lanes 1 and 2), empty plasmid pVAX-1 (lanes 3 and 4), and phosphate buffered saline (PBS) (lanes 5 and 6) are shown. Western blot for sera from all 12 mice were performed individually but only two strips for each group are presented. The positions and molecular masses (in kilodaltons) of protein standards are also shown.