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. 2013 May 1;88(5):883–887. doi: 10.4269/ajtmh.12-0727

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Figure 5. — The in vitro proliferation of splenocytes from mice vaccinated with ROP8-pVAX-1 after stimulation with total lysate antigen (TLA). Splenocytes from mice immunized with pVAX-1, PBS, ROP8-pVAX-1 were harvested 2 weeks after the third DNA booster. Following 72 h of stimulation with TLA, BrdU was added and then the stimulation index was calculated as the ratio of the average OD₅₇₀ value of wells containing antigen-stimulated cells to the average OD₅₇₀ value of wells containing only cells with medium.