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. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: Differentiation. 2011 Mar 17;81(4):233–242. doi: 10.1016/j.diff.2011.02.005

Figure 4.

Figure 4

1 prevents proliferation, but not survival, of Ink4a/Arf-/-; PTEN-/-; EGFRvIII astrocytes (SS05 cells). (A) Total number of cells/plate treated with different concentrations of 1 and FBS after 4 days. (B) Increasing concentrations (>40 μM) of 1 induces death of SS05 cells over time. (C) Protein blotting time-course analysis of cell cycle regulatory genes in SS05 cells treated with 1 compared to vehicle control. (D) Cdk4 and Tuj1 protein levels in SS05 cells treated with vehicle or 1 for 4 days, then cultured for 3 additional days after washout of drug, in the presence of 1% FBS. Gapdh served as a normalization control. (Data in graphs represent ± s.d. from three independent experiments).