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. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: Differentiation. 2011 Mar 17;81(4):233–242. doi: 10.1016/j.diff.2011.02.005

Figure 5.

Figure 5

Ink4a/Arf-/-; PTEN-/-; EGFRvIII astrocytes (SS05 cells) display an irreversible block in proliferation with 1 treatment. (A) Quantification of the total number of cells/field after vehicle or 1 pre-treatment is shown. (B-D) Shown are representative images of SS05 cells treated with 1 for 4 days, pre-treated with vehicle for 4 days and post-treated with 10% FBS for 4 days, or pre-treated with 1 for 4 days and post-treated with 10% FBS for 4 days. Note in (D): 1 pre-treated cultures and post-treated with FBS contain a mixture of Ki67+ cells with small round nuclei (arrowhead) and Ki67- cells with large flat nuclei (arrow). Scale bar: 10 μm. (E) Quantification of the percentage of Ki67+ cells is shown. (F) Quantification of the percentage of clones expressing Tuj1 is shown. (*p < 0.05 or **p < 0.001, t-test, data in graphs represent ± s.d. from three independent experiments).