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. 2013 Jun 5;88(6):1028–1031. doi: 10.4269/ajtmh.12-0571

Table 1.

Results of microscopy with the numbers of S. haematobium eggs/10 mL urine divided into egg counts of 1–49 and > 50 eggs/10 mL indicative of low and high intensity of infections respectively and real-time PCR results divided into high (Ct < 30), moderate (30 ≤ Ct ≥ 35), and low (Ct > 35) DNA loads and negative (no amplification detected in 50 cycles)*

PCR Ct Values
Negative Ct > 35 30 > Ct < 35 Ct < 30 Total Median Ct-value in PCR (range)
Microscopy egg class
 Negative 571 (84.6%) 56 (8.3%) 21 (3.1%) 25 (3.7%) 673 (100%) 35.3 (20.5–39.4)
 1–49 eggs/10 mL 7 (14.8%) 3 (6.3%) 9 (19.1%) 28 (59.6%) 47 (100%) 25.8 (17.8–39.1)
 ≥ 50 eggs/10 mL 0 (0%) 0 (0%) 0 (0%) 10 (100%) 10 (100%) 23.1 (16.1–28.1)
Total 578 (79.2%) 59 (8.1%) 30 (4.1%) 63 (8.6%) 730 (100%) 31.9 (16.1–39.4)
*

PCR = polymerase chain reaction; Ct = cycle-threshold.