Table 1.
Comparative Ct values in real-time PCR by using five extraction methods for isolation of Strongyloides DNA in stool samples*
| Extraction method | No. S. ratti third-stage larvae spiked in stool (no. positive/no. tested) | ||
|---|---|---|---|
| 10 | 5 | 1 | |
| PowerSoil kit | 25 (4/4) | 28 (4/4) | 28 (4/4) |
| Fecal DNA kit | 27 (4/4) | 27 (4/4) | 30 (3/4) |
| QIAamp Tissue kit (modified) | 28 (4/4) | 30 (4/4) | 30 (4/4) |
| RBB+C | 34 (2/4) | 36 (1/4) | 36 (2/4) |
| RBB+NeM | 31 (4/4) | 32 (4/4) | 32 (3/4) |
*
PCR = polymerase chain reaction; Ct = cycle threshold; RBB+C = repeated bead beating plus column method; RBB+NEM = semi-automated DNA extraction method using repeated bead beating combined with NucliSENS easyMAG. Values in parentheses are number of real-time PCRs detected in quadruplicate samples,