. 2013 Jun 5;88(6):1052–1061. doi: 10.4269/ajtmh.12-0484

Table 2.

Species and SNP-specific primers for wild-type plasmids of the three STH β-tubulins^*

Codon	Species	Primer sequences (5′–3′)	T°ann (°C)
167	A. lumbricoides	Forward: ACTCGCTTGGTGGAG	58.4
	A. lumbricoides	Reverse: CAACCAACTGATGAACGGAC	58.4
	T. trichiura	Forward: CCTCGGCGGCGGAACTG	56.4
	T. trichiura	Reverse: GGTGGACTGACAGAGTTGC	56.4
	N. americanus	Forward: TGACTGTCTCCAGGTAATTCG	58
	N. americanus	Reverse: CGATGATACGCGGGATG	58
198	A. lumbricoides	Forward: CCGTGAAGAATACCCCGACA	58.7
	A. lumbricoides	Reverse: TATGTGGGATTTGTAAGCTTCAG	58.7
	T. trichiura	Forward: TTTCAGATACAGTTGTAGAACC	57
	T. trichiura	Reverse: CAAATGATTTAAGTCTCCG	57
	N. americanus	Forward: GTTTCCGACACTGTGGTTGAG	59
	N. americanus	Reverse: AGTTCGTTACTAGCCAGCTCACC	59
200	N. americanus	(same as position 198)

SNP = single nucleotide polymorphism; STH = soil-transmitted helminth; T°ann = primer annealing temperature.