Table 4.
Allele-specific primers and sequencing primers used for pyrosequencing reactions for the three STH β-tubulins
| Codon | Species | Primer sequences (5′–3′) | T°ann (°C) |
|---|---|---|---|
| 167 | A. lumbricoides | Forward: CCGTGAAGAATACCCCGAC | 59 |
| Reverse: GGTGGACTGACAGAGTTGC | |||
| Seq primer: ACCCCGACAGAATCATGAGCTCG | |||
| T. trichiura | Forward: CCTCGGCGGCGGAACTG | 59 | |
| Reverse: GGTGGACTGACAGAGTTGC | |||
| Seq primer: TGACCGAATTATGACAACT | |||
| N. americanus | Forward: CCGGATCAGGAATGGGAAC | 59 | |
| Reverse: GGCTCAACCACAGTGTCG | |||
| Seq primer: GATAGAATCATGTCCTCGT | |||
| 198 | A. lumbricoides | Forward: AGGTTTCTGATGTGGTGTTGGA | 59 |
| Reverse: CAAATGATTTAAGTCTCCG | |||
| Seq primer: GGTTGAGAACACCGAT | |||
| T. trichiura | Forward: AGGTTTCAGATACAGTTGTAG | 59 | |
| Reverse: CAAATGATTTAAGTCTCCG | |||
| Seq primer: GGTAGAGAACACGGACG | |||
| N. americanus | Forward: GTTTCCGACACTGTGGTTGAG | 58 | |
| Reverse: GATTCAGATCTCCATATGTTGGG | |||
| Seq primer: GTTGAGAATACAGATG | |||
| 200 | N. americanus | Forward: GTTTCCGACACTGTGGTTGAG | 58 |
| Reverse: GATTCAGATCTCCATATGTTGGG | |||
| Seq primer: GTTGAGAATACAGATG |
T° ann = primer annealing temperature; Seq primer = sequencing primer.