Table 5.

Conditions applied to each egg hatch assay

	End point (hrs)	Solutions	Storage type and temperature
Experiment A*	18	N/A	N/A
	45	N/A	N/A
Experiment B-1†	18	N/A	Anaerobic in zip-loc bag at RT§
Experiment B-2†	18	N/A	Anaerobic in air tight container at RT§
Experiment B-3†	18	N/A	Anaerobic in air tight container at 4°C
Experiment C†	18	50% Lugol's iodine¶ + 20% sodium thiosulfate¶	Anaerobic in air tight container at RT
Experiment D†	18	Amphotericin B∥+ 50% Lugol's iodine + 20% sodium thiosulfate	Anaerobic in air tight container at RT
Experiment E‡	16	Amphotericin B + 50% Lugol's iodine + 20% sodium thiosulfate	N/A

^*Experiment A was split into two assays (A-1 and A-2), in experiment A-1 eggs were recovered from feces of dogs infected with laboratory isolates of Ancylostoma caninum (TRS strain), whereas in experiment A-2, eggs were recovered from feces of dogs infected with field isolates of A. caninum.

^†Eggs recovered from stool were stored under anaerobic conditions before the assays.

^‡Eggs were recovered from fresh stool samples before the assays.

^§The temperature given refers to the storage temperature of the feces and not the incubation temperature of the assay.

^¶Staining and distaining solutions applied into wells at the end of the assay to facilitate the scoring.

^∥Antifungal solution added to the egg solution before the assay.

In assays from all experiments drug concentrations tested were from 0.8 to 0.003125 μg/mL.

RT = room temperature; N/A = not applicable.