Table 1.

Primers and probe sequences used in the heteroduplex tracking assay (HTA) and Pvmdr1 assays

Genetic marker	Primer/probe sequence	Amplification program
Pvmsp1
HTA forward*	5′-GATGGTCCTCAAAAGGGAAA	95°C × 15′; 35 cycles of
HTA reverse*	5′-GACATGCGTAAGCGGATTTT	94°C × 1′, 55°C × 2′, and 72°C × 2′; 72°C × 5′
Pv aldolase
Forward	5′-GACAGTGCCACCATCCTTACC
Reverse	5′-CCTTCTCAACATTCTCCTTCTTTCC
Probe	5′-VIC-ATTCCATCAATGCGTTAGGC-MGB
Pvmdr1
Copy number forward	5′-CGATTGAAGATTCCGAAGTTG	50°C ×2′; 95°C × 10′; 40
Copy number reverse	5′-TTAGAACCCACCAGCGTTTC	cycles of 95°C × 15″ and
Copy number probe	5′-6FAM-GAAGGTGCTGATCCACGATT-MGB	60°C × 1′
Sequencing forward	5′-ATAGTCATGCCCCAGGATTG	95°C × 15′; 35 cycles of
Sequencing reverse	5′-CCTTTCGAAGGACAGCTTTG	95°C × 15″, 55°C × 30″,
		and 72°C × 1′; 72°C × 10′

^*Primers modified from Maestre and others.²⁸