Hemolytic index calculation. A scatter plot was created with the moles of drug/kg (Log2) of test drugs on the x axis, and percentage of red block cell (RBC) hemolysis on the y axis. The percentage of RBC loss induced by primaquine given at ED100 was defined as 100% hemolysis. The percent RBC hemolysis observed in animals administered test compounds was direct compared with a standard curve of primaquine at equimolar doses. •, Primaquine; □, pamaquine; ⋄, chloroquine; △, mefloquine.