FIG. 2.

Northern blot analysis of bpeB mRNA expression in B. pseudomallei KHW, KHWΔbpeAB, and KHWΔbpeAB(pUCP28TbpeAB). Total RNA was extracted with the TRIzol reagent (Invitrogen, Carlsbad, Calif.). Total RNA (10 μg) was resolved on a 1% formaldehyde-agarose gel, transferred onto a nitrocellulose membrane, and probed with a ³²P-radiolabeled partial bpeAB PCR product generated with AcrA3′F3-AcrB5′R3 according to the instructions of the manufacturer (Amersham) (Fig. 1; Table 2). Lane 1, a mixture of 0.5 μg each of full-length bpeAB, bpeB, and 16S RNA PCR products included as a positive control; lanes 2 to 4, 10 μg each of total RNA from KHWΔbpeAB, KHW, and KHWΔbpeAB (pUCP28TbpeAB), respectively.