TABLE 1.

Normalized viral spread indices (SI) and percent inhibition of viral spread of 2′-FdC and Ara-C treatments measured in dissemination assays between Vero-BV and Vero cells^a

Treatment (concn)	Normalized SIb	Inhibition (%)
Untreated	100	0
2′-FdC (5 μM)	12.21 ± 0.39	87.8 ± 0.4
2′-FdC (10 μM)	7.79 ± 2.11	92.2 ± 2.1
2′-FdC (20 μM)	9.44 ± 2.03	90.6 ± 2
Ara-C (5 μM)	3.77 ± 0.82	96.2 ± 0.8

^aValues reflect the means of four independent experiments (± standard deviation).

^bViral spread index for viral spread was calculated using the following formula: [(number of double-positive cells/total number of cells positive for CFDA)/number of cells single positive for BDV-N antigen] × 100. Thereafter, spread indices were normalized to the value obtained in the untreated cell control for each experiment.