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Treatment of 10T1/2 cells with Wnt ligands increases αSMA expression. (A) Axin2 transcript expression decreases after treatment of cells with TGF-β and increases after treatment with Wnt4 or Wnt3a. (B) Wnt ligands can increase expression of αSMA in 10T1/2 cells. Analyzed by t test. *P<0.05; **P<0.01; ***P<0.001. Samples done in triplicate and repeated twice.
