Table 2.

Absolute copy numbers of mRNA in urinary cells per 1 μg total RNA

Group, Patient No.	CD3	CD20	CD25	CD103	CXCR3	IP10	MIG	PERF	GB	PI9	IL2	IFN-r	IL4	IL10	TGFB1	FOXP3	CTLA4	TLR5	BK Virus VP1	18S rRNA Copies per 1 μg Total RNA
Tolerant
2	47,997	746	798	653	4497	1308	1255	1904	1853	19,767	193	333	0	1028	99,225	640	3460	5475	0	5.18E+08
5	28,250	569	1452	2301	10,863	6317	20,495	4800	2818	3727	0	386	0	356	25,107	1153	91	1195	0	2.14E+08
6	3003	398	201	664	3706	157	451	854	842	3087	0	0	0	479	15,963	188	29	1121	0	1.22E+08
8	6798	5136	190	144	2192	1518	12,002	1261	441	3604	13	95	13	102	4414	97	283	3092	0	2.57E+09
9	24,546	1561	604	304	7775	2387	7244	3826	1371	3105	0	42	0	551	22,760	1898	256	2178	0	4.29E+08
Mean	22,119	1682	649	813	5807	2337	8289	2529	1465	6658	41	171	3	503	33,494	795	824	2612	0	7.72E+08
SEM	8103	886	232	385	1559	1057	3707	763	414	3280	38	79	3	152	16,821	334	661	802	0	4.56E+08
Nontolerant
1	64,091	12,963	5837	784	10,692	2438	8020	7430	2825	58,721	90	350	230	5004	107,235	2994	3198	6892	0	7.26E+08
3	5961	3889	2255	88	522	64	458	1421	863	2983	60	13	13	179	8924	613	169	1536	0	1.36E+09
4	67,001	13,913	3134	1269	16,416	24,374	99,032	10,946	12,848	37,487	341	651	0	3534	98,861	2041	2903	5811	0	1.09E+09
7	32,040	2849	1625	555	5749	2098	4513	3522	4871	9855	101	36	0	4473	70,882	1060	1051	5141	0	4.78E+08
10	49,386	751	4518	1919	10,858	6848	26,386	9566	8784	54,651	190	258	0	16,656	230,633	943	2348	13,304	0	2.40E+09
Mean	43,696	6873	3474	923	8847	7164	27,682	6577	6038	32,739	156	262	49	5969	103,307	1530	1934	6537	0	1.21E+09
SEM	11,294	2732	765	313	2680	4442	18,381	1798	2149	11,373	51	117	45	2800	36,199	437	575	1916	0	3.33E+08
Mann–Whitney
P value	0.15	0.10	0.01	0.80	0.53	0.41	0.53	0.15	0.056	0.22	0.10	0.80	0.72	0.10	0.22	0.31	0.31	0.10		0.22

Levels of mRNA were measured with the use of preamplification enhanced real-time quantitative PCR assays using gene-specific oligonucleotide pairs and TaqMan probes. Mean and SEM of the mean copy number of each mRNA measure are shown. Patients are grouped by phenotypic designation as tolerant and nontolerant because of the biopsy findings (see text and Table 1). All tolerant patients had been withdrawn from immunosuppression for ≥1 years at the time of urinary mRNA sampling (between 36 and 48 months post-transplantation), whereas all nontolerant recipients, requiring either continuing immunosuppression (patients 7 and 10) or immunosuppressive reinstitution (patients 1, 3, and 4), had samples obtained during this same interval.