Figure 2.

The preservation of tissue morphology by prior US exposure is observed 4 weeks after IRI. Mice were exposed to US 24 hours before mild (25 minutes) IRI and were maintained for 4 weeks. Mice receiving US before IRI had (A) less tubulointerstitial fibrosis (quantified using Masson trichrome staining) and (B) >85% reduction in renal mRNA expression of profibrotic genes. n=3. Data in B are the mean ± SEM for ∆CT (change in cycle threshold) values calculated on the basis of tissue glyceraldehyde 3-phosphate dehydrogenase expression. αSMA, α-smooth muscle actin; Col1, collagen 1; Col3, collagen 3; Vim, vimentin.