Figure 3.

Estimated Carbon Flux Distribution through Central Metabolism for Intracellular M. tuberculosis

Arrows point in the net flux direction, and the width of each line is proportional to the underlying flux value. Glycolysis/gluconeogenesis (TCA), oxidative pentose phosphate pathway (EMP), tricarboxylic acid cycle (TCA), anaplerotic reactions (ANA). Standard abbreviations are used for the amino acids. Metabolite abbreviations: ACCOA, acetyl-CoA; ACE, acetate; CHO, chorismate; E4P, d-erythrose 4-phosphate; F6P, d-fructose 6-phosphate; FBP, d-fructose 1,6-bisphosphate; FUM, fumarate; G6P, d-glucose 6-phosphate; GA3P, glyceraldehyde 3-phosphate; GLX, glyoxylate; GLYC, glycerol; ICIT, isocitrate/citrate; KIV, 2-oxoisovalerate; MALOAA, l-malate-oxaloacetate; OXG, 2-oxoglutarate; R5P,α-d-ribose 5-phosphate/l-ribulose 5-phosphate/l-xylulose 5-phosphate; PEP, phosphoenolpyruvate; PGA, 2-phospho-d-glycerate/3-phospho-d-glycerate; PYR, pyruvate; S7P, sedoheptulose 7-phosphate; SUC, succinate; SUCCOA, succinyl-CoA. Enzyme abbreviations: CS, citrate synthase; ENO, enolase; FBA, fructose-bisphosphate adolase; FBP, fructose-bisphosphatase; FUM:fumurase; GAPA, glyceraldehyde 3-phosphate dehydrogenase; GND, 6-phosphogluconate dehydrogenase; ICL, isocitrate lyase; ICDH, isocitrate dehydrogenase NADP-dependent; KOR/KGD, α-ketoglutarate ferredoxin oxidoreductase/α-ketoglutarate decarboxylase; MEZ/PCA, malic enzyme/pyruvate carboxylase: MDH, malate dehydrogenase; MS, malate synthase; PCK, phosphoenolpyruvate carboxykinase; PDH, pyruvate dehydrogenase; PGI, glucose phosphate isomerase; PYK, pyruvate kinase; SDH, succinate dehydrogenase; SCS, Succinyl CoA synthetase; TAL, transaldolase; TKT1/2, transketolase. Import from the macrophage is denoted as [mac], and export to biomass is labeled _bm.