Figure 4.

Isotopomer Profiles Indicate that Alanine, ASP, and GLU Are Predominantly Taken Up from Macrophages and Incorporated Directly into Biomass, whereas Valine Is Predominantly Synthesized De Novo

(A–D) Proportion of ¹³C in isotopomers of alanine (A) and valine (B) derived from macrophage (gray bars), intracellular M. tuberculosis (black bars), and M. tuberculosis grown in labeled RPMI (white bars). Isotopomers ratios for GLU (C) and ASP (D) represented as line plots. The THP-1 cells were prelabeled by passaging with uniformly labeled [U-¹³C₆] glucose prior to the infections. Closed symbols in black from intracellular M. tuberculosis protein; open symbols in gray from macrophage protein. The error bars indicate SD from 3–4 independent experiments.

See also Table S2.