FIGURE 5.

Ag-specific Ab titers in mice deficient for TLR 9. Groups of WT C57BL/6 or TLR9−/− mice (n = 3) were vaccinated s.c. with 10 μg of CpG/rWNVE, LPS/rWNVE, or −/rWNVE nanoparticles. After 4 wk, mice were bled retro-orbitally, and serum was analyzed for rWNVE-specific 1gG1 (A) and IgG2b (B) by ELISA. *p < 0.05. CpG/rWNVE, recombinant West Nile virus envelope protein encapsulated in CpG-modified nanoparticles; LPS/rWNVE, recombinant West Nile virus envelope protein encapsulated in LPS-modified nanoparticles. WT, wild-type.