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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012 Aug 9;4(6):605–619. doi: 10.1002/wnan.1186

Figure 8.

Figure 8

Fluorescence displacement assay based on ligand-conjugated QDots for the drug discovery of allosteric antidepressants. (A) Targeted hSERTs bind to the QDot-tagged ligands, forming complexes that increase fluorescent signal along the membrane. When exposed to a potential drug that induces a conformational change in the binding site, the QDot-tagged ligands are displaced resulting in a decrease in fluorescence intensity. (B) Representative time-lapse fluorescent images show the effect of paroxetine on lhigand-hSERT displacement in the presence of PBS buffer (control), 10 µM, and 20 µM paroxetine. (Reprinted with permission from Ref 62. Copyright 2011 American Chemical Society)