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Dual hydrogel systems with fluorescent-tagged immobilized MI-biomolecules. A) MI-biotin tagged with FITC-NeutrAvidin. B-D) MI-NeutrAvidin tagged with Oregon Green 488 biocytin B) and Alexa Fluor 594 biocytin (red) after two separate irradiation steps, C) in a gradient pattern using in-focus masks and D) in a gradient pattern using out-of-focus masks (scale bars = 500 μm)
