FIGURE 8.

Pam₃Cys, a TLR2 agonist, can rescue Vδ2 T cell proliferation and IFN-γ production after blocking TNF-α. A and B, Anti-TNF-α blocking Ab (5 μg/ml) or Pam₃Cys (10 μg/ml) was added to IPP/IL-2-treated cultures, and cells were cultured for 10 days. Both Vδ2 frequency (A) and absolute number (B) were detected every 3 days. The cultures were set up in triplicate. C, Purified Vδ2 T cells from primary PBMC or IPP-expanded PBMC were treated with or without anti-TNF-α blocking Abs or Pam₃Cys and stimulated by IPP. After stimulation for 18 h, the level of IFN-γ in cell-free supernatant was detected by ELISA. Data are representative of three experiments yielding similar results.