TABLE 2.
Device | Step | Problem | Possible reason | Solution |
---|---|---|---|---|
Standard FP device | 1,19 | Inconsistency between pressure curves and injury severity | Presence of air in reservoir | Level the cylindrical reservoir and open a valve centered on the top of the reservoir to remove trapped air Introduce saline from the side that is connected to the transducer tube to remove trapped air Check the operation of the device to ensure removal of air |
MFP device | 1,19 | Inconsistency between pressure curves and injury severity | Presence of air in pressure chamber | Using the locking swivel attached to the base of pressure chamber, tilt the chamber after opening the valves on top and bottom Introduce saline from the bottom of the chamber, removing air through the valve on the top Close the valve immediately Check operation of the device several times to ensure the removal of air from the system |
Air entrapment in the cylindrical reservoir (standard FP device) or the pressure chamber (MFP device) can result in inconsistency between pressure curves and degrees of injury severity. The trapped air must be removed to ensure reliability and reproducibility of injury across animals.