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. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: Nat Protoc. 2010 Aug 19;5(9):1552–1563. doi: 10.1038/nprot.2010.112

TABLE 2.

Troubleshooting table.

Device Step Problem Possible reason Solution
Standard FP device 1,19 Inconsistency between pressure curves and injury severity Presence of air in reservoir Level the cylindrical reservoir and open a valve centered on the top of the reservoir to remove trapped air
Introduce saline from the side that is connected to the transducer tube to remove trapped air
Check the operation of the device to ensure removal of air
MFP device 1,19 Inconsistency between pressure curves and injury severity Presence of air in pressure chamber Using the locking swivel attached to the base of pressure chamber, tilt the chamber after opening the valves on top and bottom
Introduce saline from the bottom of the chamber, removing air through the valve on the top
Close the valve immediately
Check operation of the device several times to ensure the removal of air from the system

Air entrapment in the cylindrical reservoir (standard FP device) or the pressure chamber (MFP device) can result in inconsistency between pressure curves and degrees of injury severity. The trapped air must be removed to ensure reliability and reproducibility of injury across animals.