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. Author manuscript; available in PMC: 2014 Jun 18.
Published in final edited form as: Cell Physiol Biochem. 2013 Jun 18;31(6):925–937. doi: 10.1159/000350111

Fig. 3.

Fig. 3

Simvastatin induces autophagy in CAMs via inhibition of Rac1 and mTOR. (A) and (B) Mouse CAMs were treated with simvastatin (Simva, 5 μM) in the presence or absence of mevalonate (Mev, 10 μM), farnesol (Far, 10 μM), or geranylgeraniol (Ger, 10 μM). Representative Western blot gel document and summarized data show the relative expression of GTP-bound active Rac1 or total Rac1. (C) and (D) Mouse CAMs were treated simvastatin (Simva, 5 μM), or NSC (Rac1 inhibitor, 30 μM), or transfected with Rac1 cDNA and then treated with simvastatin. Panel C shows the summarized data for the activity of mTOR. Panel D shows the summarized data for the percentage of cells with autophagy using Cyto-ID probes. * P<0.05 vs. Ctrl; # P<0.05 vs. Simva (n=4).