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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Genesis. 2013 Jun 24;51(8):587–595. doi: 10.1002/dvg.22401

Figure 3. CXCR2 is stably deleted by poly(I:C) in Mx-Cre::Cxcr2 CKO mice.

Figure 3

(A) Efficient deletion of CXCR2 after poly(I:C) injection. Peripheral blood cells from Cxcr2fl/+::Mx-Cre+ and Cxcr2fl/fl::Mx-Cre+ mice before injection (figure 3A, top) and 4 wks after injection (figure 3A, bottom), were stained with Ly6GFITC and CXCR2PE antibodies and were analyzed by flow cytometry. These data represent at least three independent experiments. (B) CXCR2 deletion is time dependent and irreversible. Peripheral blood cells collected from Cxcr2fl/fl::Mx-Cre+ mice before injection, at 2 wks, 3 wks, 4 wks, and 18 wks after poly(I:C) injection, were stained with Ly6GFITC and CXCR2PE antibodies and were analyzed by flow cytometry. The percentage indicated in the figures is CXCR2 positive neutrophils in the total peripheral blood cells. These data represent at least three independent experiments.