TABLE 3.
erm(B) upstream sequences from ketolide-resistant S. pyogenes isolates
| Isolate | Sequence |
|---|---|
| pAM77 | TTTTGT--ATAATAGGAATTGAAGTTAAA |
| Tn1545 | GT |
| MSR 63 | -- |
| MSR 610 | -- |
| pAM77 | TTAGATGCTAAAAATTTGTAATTAAGAAggagg |
| Tn1545 | |
| MSR 63 | |
| MSR 610 | |
| pAM77 | GATTCGTCATGTTGGTATTCCAAATGCGTA |
| Tn1545 | |
| MSR 63 | |
| MSR 610 | |
| pAM77 | ATGTAGATAAAACATCTACTGTTTTGAAACAG |
| Tn1545 | |
| MSR 63 | |
| MSR 610 | |
| pAM77 | ACTAAAAACAGTGATTACGCAGATAAA |
| Tn1545 | |
| MSR 63 | |
| MSR 610 | |
| pAM77 | TA----CGTTAGATTAATTCCTACCAGTGACT |
| Tn1545 | AATA |
| MSR 63 | AATA |
| MSR 610 | AATA |
| pAM77 | AATCTTATGACTTTTTAAACAGATAACTAAAA |
| Tn1545 | |
| MSR 63 | |
| MSR 610 | |
| pAM77 | ATTACAAACAAATCGTTTAACTTCTGTATTTA |
| Tn1545 | |
| MSR 63 | |
| MSR 610 | G |
| pAM77 | TTTATAGATGTA-TCACTTCAGGAGTGATTAC |
| Tn1545 | CAA |
| MSR 63 | AA |
| MSR 610 | AA |
a
erm(B) upstream sequences from ketolide-resistant S. pyogenes isolates are shown. The sequences upstream of erm(B) in Streptococcus sanguis (pAM77), S. pneumoniae (Tn1545), and the two ketolide-resistant S. pyogenes strains are compared (15). Boldface residues delineate the promoter region, lowercase letters represent the RBS before a leader peptide (underlined) of 36 amino acids (pAM77) or 27 amino acids (Tn1545, MSR 63, MSR610). (TAA is a stop codon.) Only residues that differ from pAM77 erm(B) are shown.