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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Curr Protoc Mol Biol. 2012 Oct;0 12:Unit–12.15. doi: 10.1002/0471142727.mb1215s100

Figure 2. Overview of ligation strategy used to join together DNA encoding TALE repeats.

Figure 2

A fragment of DNA encoding carboxy-terminal TALE repeat(s) created by digestion with BbsI and BamHI restriction enzymes is ligated to a vector backbone of DNA encoding amino-terminal TALE repeat(s) created by digestion with BsaI and BamHI. Ligation occurs via compatible DNA overhangs created by the TypeIIS enzymes BbsI and BamHI. The resulting plasmid can be used in subsequent iterative ligations of the same type shown here.