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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Curr Protoc Mol Biol. 2012 Oct;0 12:Unit–12.15. doi: 10.1002/0471142727.mb1215s100

Figure 4. Example of a customized graphical guides generated by ZiFiT Targeter for construction of an engineered TALEN.

Figure 4

This particular guide illustrates the construction of a TALE array consisting of 15.5 repeats. TALE repeats are represented as colored rectangles with the identities of their RVDs shown in two letter code within the rectangle. Bases bound by each TALE repeat are shown beneath the rectangle. Ligations of units to be performed are indicated by black lines. The numbers of plasmids used to perform the initial ligations are shown above the first row. The final assembled array is cloned into the pJDS-series TALEN expression vector indicated at the bottom. Cloning into this vector adds the required carboxy-terminal 0.5 TALE repeat domain and fuses the assembled TALE repeat array to the wild-type FokI nuclease domain, thereby generating a TALEN expression plasmid. A) Graphic for TALEN assembled using REAL B) Graphic for TALEN assembled using REAL-Fast