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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Curr Protoc Mol Biol. 2012 Oct;0 12:Unit–12.15. doi: 10.1002/0471142727.mb1215s100
Component Amount
Purified vector backbone (from step 23) 1 µl
Purified fragment (from step 25) or water 3 µl
Quick Ligase Buffer (NEB) 4.5 µl
T4 DNA Ligase (400U/ul) 0.5 µl
Total 9 µl