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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Curr Protoc Mol Biol. 2012 Oct;0 12:Unit–12.15. doi: 10.1002/0471142727.mb1215s100
Component Amount
Plasmid 0.5 µg
10X Buffer (NEBuffer 4) 5 µl
Bovine Serum Albumin (10 mg/ml) 5 µl
KpnI-HF (20 U/µl) 1 µl
BamHI (20 U/µl) 1 µl
Add nuclease-free water to a total volume of 50 µl