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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2013 Aug;72(8):768–781. doi: 10.1097/NEN.0b013e31829d8d9d

Figure 4.

Figure 4

Analysis of retinal ganglion cell (RGC) number following traumatic axonal injury (TAI). (A) Images from side to side of the retinal section immunostained with anti-Brn3a antibody. (B) Placement of a grid (0.435 mm x 0.115 mm = 0.05 mm2) expanding along the image parallel to the alignment of the RGCs and covering all RGCs that were counted. (C) Quantitative assessment reveals a subtle time-dependent decrease of RGCs in both sham and injured animals from 7 days to 14 days and 28 days post-injury (* p < 0.05, # p < 0.05). At each time point there is no difference in numbers of RGC between the sham and the injured groups. Scale bars: A, 500 μm; B, 200 μm.