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. 2004 Apr;48(4):1300–1306. doi: 10.1128/AAC.48.4.1300-1306.2004

FIG. 2.

FIG. 2.

Pre-steady-state kinetics of incorporation of dCMP (•) (2.5 μM), (+)FTC-MP (⧫) (2 μM), and (−)FTC-MP (▪) (100 μM) into a DNA-DNA 23-mer/45-mer primer-template by HIV-1 RT. (A) The solid lines represent the best fit of the data (•) to a burst equation with an amplitude A of 126 ± 1 nM, an observed first-order rate constant for the burst phase kobsd of 25.7 ± 0.6 s−1, and an observed rate constant for the linear phase kss of 0.027 ± 0.004 s−1 for dCMP incorporation. (B) The solid lines represent the best fit of the data (⧫) to a burst equation with an A of 48 ± 2 nM, kobsd of 0.60 ± 0.05 s−1, and kss of 0.043 ± 0.004 s−1 for (+)FTC-MP incorporation. (C) The solid line represents the best fit of the data (▪) to a linear equation, y = ax + b, with a slope of 0.39 nM · s−1 and an observed rate of 0.0039 s−1.