Table 2.

(A) Kinetic parameters for wild-type IAPP, single aromatic to Leu mutants, and F15 variants amyloid formation in the absence of HFIP. (B) Comparison of kinetic parameters normalized with respect to wild-type for single aromatic to Leu mutants, and F15 variants in the presence and absence of HFIP. Experiments were conducted at 25 °C, pH 7.4 20 mM Tris-HCl in the presence and absence of HFIP.

(A) peptide	wild-type	F15L	F23L	Y37L	F15NLe	F15I	F15TLe
Lag time (hr)	16±1.3a	9.8±0.1	38±0.6	42±2.3	20±1.7	41±2.3	267±4
t50 (hr)	23±0.9	11±0.1	42±1.7	63±2.8	31±1.3	61±5.8	276±6

(B) mutant	HFIP	F15L	F23L	Y37L	F15NLe	F15I	F15TLe
Lag time/wild-type Lag time	present	0.4 ±0.08b	1.7±0.4	2.1±0.5	1.9±0.4	2.3±0.5	22±5
t50/t50 of wild-type	present	0.4±0.08	1.7±0.3	2.8±0.5	1.8±0.4	2.9±0.6	28±6
Lag time/wild-type Lag time	absent	0.6±0.08	2.4±0.2	2.6±0.3	1.3±0.1	2.6±0.3	17±1.4
t50/t50 of wild-type	absent	0.5±0.02	1.8±0.1	2.7±0.2	1.3±0.1	2.7±0.3	12±0.5

^aThe quoted uncertainty is the standard deviation.

^bThe uncertainty was calculated by standard propagation of error.